Trifolirhizin relieves renal injury in a diabetic nephropathy model by inducing autophagy and inhibiting oxidative stress through the regulation of PI3K/AKT/mTOR pathway

Purpose: To evaluate the effects of trifolirhizin on diabetic nephropathy (DN), and mechanism action. 
 Methods: Male db/db mice (8 weeks, n = 24) age-matched control (n 6) were obtained. The further divided into four groups administered increasing doses (0, 12.5, 25 50 mg/kg). Histological analysis renal tissues performed by H & E staining. Blood urea nitrogen (BUN) creatinine determined using enzyme-linked immunosorbent assay (ELISA). Immunoblot TUNEL to investigate effect autophagy apoptosis, while ELISA dihydroethidium (DHE) staining conducted reactive oxygen species (ROS), malondialdehyde (MDA) superoxide dismutase (SOD) levels. PI3K/AKT/mTOR pathway was assays. Results: Trifolirhizin alleviated injury in mice, also activate inhibited apoptosis (p < 0.001). In addition, oxidative stress response tissue therefore relieved model Conclusion: alleviates activates autophagy, inhibits mice. Therefore, is a promising drug for treatment DN.